Search results for "Culture-dependent approach"

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Cultivable microorganisms associated with honeys of different geographical and botanical origin

2014

In this study, the composition of the cultivable microbial populations of 38 nectar honey and honeydew honey samples of different botanical and geographical origin were assessed. After growth in specific media, various colonies with different appearance were isolated and purified before phenotypic (morphological, physiological and biochemical traits) and genotypic [randomly amplified polymorphic DNA (RAPD), repetitive DNA elements-PCR (rep-PCR) and restriction fragment length polymorphism (RFLP)] differentiation. The identification was carried out by 16S rRNA gene sequencing for bacteria and, in addition to RFLP, by sequencing the D1/D2 region of the 26S rRNA gene for yeasts and the 5.8S-IT…

Filamentous fungiBacillus amyloliquefaciensMicroorganismCulture-dependent approachFlowersMicrobiologyYeastsBotanyGenotypeNectarBacteria; Culture-dependent approach; Filamentous fungi; Honey; Molecular characterisation; Yeasts; Bacteria; Discriminant Analysis; Flowers; Fungi; Geography; Honey; YeastsbiologyBacteriaGeographyHoney Microorganisms botanical originsFungiDiscriminant AnalysisHoneyRibosomal RNAbiology.organism_classificationRAPDMolecular characterisationRestriction fragment length polymorphismBacteriaFood ScienceSettore AGR/16 - Microbiologia Agraria
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